Composite

Part:BBa_K1039014

Designed by: Jama Hagi-Yusuf   Group: iGEM13_Waterloo   (2013-09-12)


PhiC31 Intgrase and Recombination Directionality Factor Expression Cassette

A strong promoter and strong RBS (BBa_K608002) drive expression of PhiC31 integrase (BBa_K1039012) and PhiC31 recombination directionality factor (RDF) (BBa_K1039013). The RBS (BBa_B0034) is associated with RDF.

Together, PhiC31 integrase and RDF (BBa_K1039012 and BBa_K1039013) catalyze site-specific recombination between PhiC31 attR and attL sites (Bba_K1039037 and Bba_K1039038).

They will also allow flipping of PhiC31 invertible promoter switches BBa_K1039011 and BBa_K1039010 from RL state to PB state.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 112
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2561
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1726
    Illegal SapI site found at 2227
    Illegal SapI site found at 2449
    Illegal SapI site found at 2575
    Illegal SapI.rc site found at 1417
    Illegal SapI.rc site found at 1475
    Illegal SapI.rc site found at 1580


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